Analysis Note

Click on the following link for a demo: Easy to Use High-Throughput Kits for Cholesterol and Lipid Extractions

General description

Cholesterol is a structural component of cell membranes and a precursor to hormones. The Cholesterol Extraction Kit enables the extraction of sterols including cholesterol from biological samples in two simple steps. The extracted sterols can then be saponified, derivatized, and quantified using gas chromatography (GC) with flame-ionization detection (FID) or gas chromatography/mass spectrometry (GC/MS).

Other Notes

Easy to Use High-Throughput Kits for Cholesterol and Lipid Extractions. Watch the Video to learn more and to see how the technology works.

Principle

Sterols including cholesterol are typically extracted using a dual solvent partition system containing a lipophilic solvent and an aqueous solvent. Sterols are retained in the lower chloroform phase, whereas, aqueous-soluble compounds are retained in the methanol-water layer. The sample is then centrifuged and the bottom chloroform layer is transferred with a pipette to another test tube. An aliquot of the transferred layer is then saponified with methanolic NaOH or KOH, and then derivatized with trimethylsilyl chloride (chlorotrimethylsilane). The formed cholesterol-TMS ester is reconstituted with hexane and injected directly into a GC-FID or GC/MS system for quantification.The Cholesterol Extraction Kit shortens the extraction process by eliminating the need to prepare solvents and standards, centrifugation, and pipetting. Once the sample is homogenized and dissolved in the Extraction Solvent containing the internal standard, it is inverted twice and poured into the syringe containing a filter, which preferentially elutes the chloroform layer containing sterols. A portion of the total sterol extract can be saponified and derivatized for GC-FID or GC/MS analysis as described in the procedure.We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods.Find details here.

Suitability

This kit is suitable for the extraction of cholesterol and cholesterol-like compounds (sterols) from whole blood, serum, biological tissue and food samples.